Study on original plant species and geographical distribution of Fructus Aurantii / 中国中药杂志

The original plant species of Fructus Aurantii are multitudinous and complex, and their requirements to the growing environment is strict. In order to clarify the original plant species and geographical distribution of Fructus Aurantii which recorded in the standards and circulated, used in commodity. The national and local standards of Chinese medicinal materials were collected and the original plants of Fructus Aurantii recoded in standards were found. Ten original plant species of Fructus Aurantii (including varieties of cultivars, the same below) were recorded in the Chinese pharmacopoeia and six local standards of Zhejiang, Yunnan and Guizhou etc. The producing areas and commodity in markets of Fructus Aurantii were investigated. The growth environment and geographical distribution of them were analyzed. There are six types of Fructus Aurantii i.e., Fructus Aurantii Chuan, Fructus Aurantii Xiang, Fructus Aurantii Jiang, Fructus Aurantii Qu, Fructus Aurantii Su, Fructus Aurantii Wen, and nineteen species of original plants in the practical commodities. There are four major Fructus Aurantii producing areas: Sichuan Basin, Dongting Lake Plain, Poyang Lake Plain, Jinqu Basin and its surrounding hilly areas. All of them are located in the area of the east longitude 104° to 121° and the northern latitudes 27° to 31°. There is a certain difference between the actual commodity and the standards of medicinal materials. It is suggested that the traditional mainstream types of Fructus Aurantii with fine quality should be accepted into Chinese Pharmacopoeia, and the types with poor quality should be withdrawn from Chinese Pharmacopoeia.

Simultaneous determination for contents of naringin, hesperidin, and neohesperidin in flower of Citrus changshan-huyou by RP-HPLC / 中草药

Objective: To establish a method for the simultaneous determination for the contents of naringin, hesperidin, and neohesperidin in the flower of Citrus changshanhuyou by RP-HPLC. Methods: The HPLC method was adopted with Agilent Zorbax SB-C18 (250 mm × 4.6 mm, 5 μm) column; The mobile phase was acetonitrile-0.2% aqueous phosphoric acid (15:85) with the flow rate of 1.0 mL/min. The detection wavelength was 284 nm and column temperature was 40°C; The peak areas were quantified by external standard method. Results: According to the method, naringin linear was within 28.45-284.5 ng, the regression equation was Y = 1725.2X + 6.383 3 (r = 0.9998), and the average recovery rate was 98.83%. Hesperidin linear was within 18.09-180.93 ng, Y=1764.6 X+0.4167 (r = 0.9998) with the average recovery rate of 99.05%. Neohesperidin linear was within 85.86-858.55 ng, Y = 1914.7X + 15.892 (r = 0.9996), and the average recovery rate was 98.83%. According to the established method, the contents of sample, naringin, hesperidin, and neohesperidin were respectively determined. Conclusion: The method is simple, rapid, accurate, and reliable, which provides the reference for the quality control for the flower of C. changshanhuyou, and is suitable for the content determination.